HER2 STATUSINI ANIQLASH UCHUN IMMUNOGISTOKIMYO VA REAL VAQTDAGI PZR USULLARINI SOLISHTIRISH
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HER2, SYBR Green, ko’krak bezi saratoni, PZR##article.abstract##
Ushbu tadqiqot maqsadi intterkalirlovchi SYBR Green yashil bo'yoqlari yordamida polimeraza zanjiri reaktsiyasining (PZR) asosida ko'krak bezi saratoni hujayralarida HER2 geni (inson epidermal retseptorlari) miqdorini aniqlashdan iborat. Eksperimentlar ko'krak bezi saratonining immunogistoximiya usuli bilan teksirilgan 8 ta namunasida o'tkazildi. Ko'krak bezi saratoni namunalarida immunogistoximiya va SYBR Green rv-PZR natijalarini taqqoslash natijasi ko’rsatkichi 87,5% ga teng. Natijalar shuni ko'rsatadiki, real vaqtda PZR SYBR Green o'simta to'qimasida onkomarkerining yo'qligi haqida yolg'on xulosalarning minimal darajaga yetkazishga imkon beradi.
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