СРАВНЕНИЕ МЕТОДОВ ИММУНОГИСТОХИМИЯ И ПЦР В РЕАЛЬНОМ ВРЕМЕНИ ДЛЯ ОПРЕДЕЛЕНИЯ СТАТУСА HER2 АННОТАТЦИЯ
Ключевые слова:
HER2, SYBR Green,рак молочной железы, ПЦРАннотация
Это исследование направлено на проведение количественной полимеразной цепной реакции (ПЦР) с использованием интеркалирующего зеленого красителя, для определения количества гена HER2 (эпидермальный рецептор человека) при раке молочной железы. Эксперименты проводились на 8 подтвержденных образцах рака молочной железы. В образцах рака молочной железы сравнение результатов метода иммуногистохимии (ИГХ) и SYBR Green РВ-ПЦР идентично на 87,5%. Полученные результаты показывают, что ПЦР в реальном времени с SYBR Greenом позволяет получить минимальное количество ложноотрицательных заключений об отсутствии маркера экспрессии в опухолевой ткани.
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