MODULATING EFFECT OF CRAMBE KOTSCHYANA EXTRACT ON THE METABOLIC-FUNCTIONAL STATE OF THE LIVER IN RATS WITH EXPERIMENTAL THYROTOXICOSIS
Keywords:
experimental thyrotoxicosis, Crambe kotschyana extract, mercazolil, hepatodysfunctionAbstract
The thyroid gland and liver are intricately interrelated under both physiological and pathological conditions. Liver pathology in thyrotoxicosis is quite common (15–79%) and manifests as increased serum activity of cytolytic and/or cholestatic enzymes.
The aim of the study was to study the metabolic and functional state of the liver in animals with experimental thyrotoxicosis against the background of the administration of Crambe kotschyana Boiss extract in comparison with mercazolil.
Materials and methods: Male rats (160-180 g) with thyrotoxicosis induced by oral administration of levothyroxine sodium at a dose of 50 mcg/kg were used in the experiment. Levels of free triiodothyronine, free tetraiodothyronine, and thyroid-stimulating hormone were determined in the animals' blood serum, as well as the activity of alanine and aspartate aminotransferase enzymes, alkaline phosphatase and the total protein content, the content of glycogen and malondialdehyde was determined in the liver tissue.
Results: Experimental thyrotoxicosis was accompanied by severe hepatic dysfunction. This was confirmed by a significant increase in ALT, AST, and alkaline phosphatase activity, an increase in MDA levels, and a decrease in glycogen and total protein levels, reflecting the development of cytolysis, cholestasis, and increased oxidative stress. Under these conditions, Crambe kotschyana extract had a milder and more physiological effect on liver tissue, more effectively reduced the manifestations of oxidative stress and contributed to better restoration of liver energy reserves compared to mercazolil, which, despite partial correction of the disorders caused by thyrotoxicosis, showed its own hepatotoxicity.
Conclusion: The obtained data show pronounced liver disorders under thyrogenic hypermetabolic load and allow us to compare the corrective effects of C.kotschyana extract with the traditional thyreostatic agent, mercazolil.
References
Abegunde A. O., Adeniyi A. F., Alatise O. I. Methimazole-Induced Agranulocytosis and Cholestasis. // Case Reports in Endocrinology. — 2016. — Article ID 1529817. — DOI: 10.1155/2016/1529817. (https://www.endocrinologydiabetes.org/article/S2376-0605%2820%2930580-0/fulltext)
Bueverov A.O., Bogomolov P.O., Nechayeva O.A., Zilov A.V. Cause-and-effect relationship between thyroid and liver diseases. Meditsinskiy sovet = Medical Council. 2021;(15):88–94. (In Russ.) https://doi.org/10.21518/2079701X-2021-15-88-94.
Galanty, A.; Grudzin´ska, M.; Paz´dziora, W.; Słu˙zały, P.; Pas´ko, P. Do Brassica Vegetables Affect Thyroid Function?—A Comprehensive Systematic Review. Int. J. Mol. Sci. 2024, 25, 3988. https://doi.org/ 10.3390/ijms25073988
LiverTox. Methimazole. — In: LiverTox: Clinical and Research Information on Drug-Induced Liver Injury. National Institute of Diabetes and Digestive and Kidney Diseases. — Bethesda (MD): NCBI Bookshelf, 2023. https://www.ncbi.nlm.nih.gov/books/NBK548406.
Назарова Е.А., Тертичная Ю.М., Савина А.А. Перспективы создания лекарственных средств на основе растительного сырья, обладающего тиреотропным действием // Вопросы биологической, медицинской и фармацевтической химии. -2014. -№ 5. -С. 56−58.
Okhunov, I.I., Aripova, S.F., Bobakulov, K.M. et al. Alkaloids and other low-molecular-weight metabolites from Crambe kotschyana. Chem Nat Compd 47, 671–672 (2011). https://doi.org/10.1007/s10600-011-0030-2
Piantanida E., Ippolito S., Gallo D., Masiello E., Premoli P., Cusini C. et al. The interplay between thyroid and liver: implications for clinical practice. J Endocrinol Invest. 2020;43(7):885–899. https://doi.org/10.1007/s40618-020-01208-6.
Сергалиева М.У., Абдулкадырова Э.И., Ясенявская А.Л. Экспериментальные модели патологий щитовидной железы // Астраханский медицинский журнал Том 15, № 1, 2020 .-С.98-106.
Shibl Ramadan Samaha. Acute Exercise Tolerance in Experimentally-Induced Hyperthyroidism in Adult Male Albino Rats.// Al-Azhar Medical Journal 2016 Vol.45 Issue 2, pp.277-286.
Suzuki N., Noh J., Ito K., et al. Antithyroid drug‐induced severe liver injury in new patients with Graves’ disease in Japan. // Thyroid. - 2019. - Vol. 29, No. 3. — P. 403–410. — DOI: 10.1089/thy.2018.0457. (https://www.thyroid.org/patient-thyroid-information/ct-for-patients/february-2020/vol-13-issue-2-p-5-6)
Zeng B., Yuan L., Chu J., Yang Y., Lin S. Challenges in early identification of causes and treatment of cholestasis in patients with hyperthyroidism: a case report and literature review. J Int Med Res. 2019;48(3):300060519891018. https://doi.org/10.1177/0300060519891018.
