EKSPERIMENTAL ANTIGLAUKOMATOZ JARROHLIKDA KARBOKSIMETILTSELLYULLOZANING NATRIYLI TUZI ASOSIDAGI GIDROGELNI QO‘LLANILGANIDA REPARATIV REGENERATSIYANING IMMUNOGISTOKIMYOVIY TAVSIFI
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glaukoma, operatsiyadan keyingi fibroz, Na-KMS, immunogistokimyo##article.abstract##
Maqsad. Eksperimental antiglaykomatoz jarrohlikda natriy karboksimetilsellyuloza (Na-KMS) asosidagi gidrogelning antifibroz samaradorligini immunogistokimyoviy (IGK) markerlar - Ki-67 va desmin yordamida baholash.
Materiallar va metodlar. Tajriba 36 ta quyon (72 ta ko‘z) da o‘tkazildi, ular uch guruhga bo‘lindi: nazorat guruhi (baryer materialisiz), taqqoslovchi guruh (“Omnivisk” viskoelastigi) va asosiy guruh (Na-KMS gidrogeli). Gistologik va IGK tadqiqotlar antiglaykomatoz chuqur sinus-trabekuloiridektomiyadan keyingi belgilangan muddatlarda o‘tkazildi. Ki-67 va desminning ekspressiya darajalari hujayra proliferatsiyasi va miofibroblastik transformatsiyani baholash uchun ishlatildi.
Natijalar. Nazorat guruhida Ki-67 va desmin ekspressiyasi yuqori darajada (67% gacha) bo‘lib, faol fibroz jarayonlarini ko‘rsatdi. Na-KMS qo‘llanilishi ushbu markerlar ekspressiyasini fiziologik me’yor darajasigacha kamaytirdi (Ki-67: 20,0±2,0%; desmin: 12,5±1,5%), bu yallig‘lanish va fibroz faolligining samarali bostirilganligini ko‘rsatdi.
Xulosa. Na- KMS asosidagi gidrogel kuchli antifibroz ta’sirga ega bo‘lib, glaukomani jarrohlik yo‘li bilan davolashda toksik antifibrotik vositalarga xavfsiz alternativ sifatida ko‘rib chiqilishi mumkin.
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