IMMUNOHISTOCHEMICAL CHARACTERIZATION OF REPARATIVE REGENERATION USING HYDROGEL BASED ON THE SODIUM SALT OF CARBOXYMETHYLCELLULLOSE IN EXPERIMENTAL ANTIGLAUCOMATOUS SURGERY
Keywords:
glaucoma, postoperative fibrosis, Na-CMC, immunohistochemistryAbstract
Objective. To evaluate the antifibrotic efficacy of sodium carboxymethylcellulose (Na-CMC)-based hydrogel in experimental antiglaucomatous surgery using immunohistochemical (IHC) markers Ki-67 and desmin.
Materials and Methods. The experiment was conducted on 36 rabbits (72 eyes) divided into three groups: control (no barrier material), comparative (Omnivisc viscoelastic), and experimental (Na-CMC hydrogel). Histological and IHC examinations were performed at designated time points following deep sinus trabeculoiridectomy. Expression levels of Ki-67 and desmin were used to assess cellular proliferation and myofibroblastic transformation.
Results. In the control group, high expression levels of Ki-67 and desmin (up to 67%) indicated active fibrotic processes. The application of Na-CMC hydrogel significantly reduced the expression of both markers to near-physiological levels (Ki-67: 20.0±2.0%; desmin: 12.5±1.5%), suggesting effective suppression of inflammatory and fibrotic activity.
Conclusion. Na-CMC hydrogel demonstrates a pronounced antifibrotic effect and may serve as a safe alternative to toxic antifibrotic agents in glaucoma surgery.
References
Allyn MM, Luo RH, Hellwarth EB, Swindle-Reilly KE. Considerations for polymers used in ocular drug delivery. Front Med (Lausanne). 2022;8:787644. doi: 10.3389/fmed.2021.787644
Bisen AC. A review on polymers in ocular drug delivery systems. Med Biol Eng Comput. 2024. doi: 10.1002/mba2.77
Cai J, et al. Application of polymer hydrogels in the prevention of postoperative adhesions.
Polymers (Basel). 2023;9(2):98. doi: 10.3390/polym9020098
DSouza AA, Amiji MM. Dual-polymer carboxymethyl cellulose and poly(ethylene oxide)-based gels for the prevention of postsurgical adhesions: formulation and mechanism. J Biomed Mater Res A. 2025;113:e37852. doi: 10.1002/jbm.a.37852
Huang YC, Chen LJ, Huang YT, Chen SC, Liao YT, Lu CW, et al. Photo-crosslinked hyaluronic acid/carboxymethyl cellulose hydrogel: anti-adhesion properties and biodegradability. Int J Mol Sci. 2022;23(11):6177. doi: 10.3390/ijms23116177
Lin KT, Wang A, Nguyen AB, Iyer J, Tran SD. Recent advances in hydrogels: ophthalmic applications in cell delivery, vitreous substitutes, and ocular adhesives. Biomedicines. 2021;9(9):1203. doi: 10.3390/biomedicines9091203
Tsung TH, Tsai YC, Lee HP, Chen YH, Lu DW. Biodegradable polymer-based drug-delivery systems for ocular diseases. Int J Mol Sci. 2023;24(16):12976. doi: 10.3390/ijms241612976
Truong D, Wu KY, Nguyen L, Tran SD. Advancements in hydrogel technology for ocular drug delivery. Explor BioMat-X. 2024;1:331–352. doi: 10.37349/ebmx.2024.00023
Waldron MG, et al. Barrier materials for prevention of surgical adhesions. BJS Open.
;6(3):zrac075. doi: 10.1093/bjsopen/zrac075
Yao K, Bao Y, Ye J, Lu Y, Bi H, Wang Y. Efficacy of 1 % carboxymethylcellulose sodium for treating dry eye after phacoemulsification: results from a multicenter, open-label, randomized, controlled study. BMC Ophthalmol. 2015;15:28. doi: 10.1186/s12886-015-0005-3
