KIDNEY MORPHOLOGY IN 6-MONTH-OLD WHITE-BREED RATS IN IRON DEFICIENCY
Keywords:
iron deficiency, kidney structure, glomerular hypertrophy, tubulointerstitial remodeling, oxidative stress, morphometryAbstract
This study is devoted to the study of the effect of iron deficiency on the morphological and morphometric parameters of the kidneys of 6-month-old male white outbred rats. The experimental model used iron-deficient diet for 3 months, and general histological and morphometric examinations were performed. The results showed compensatory dilation of afferent and efferent arterioles, hypertrophy of glomerular structures, expansion of the Shumlyansky-Bowman capsule lumen, and thickening of the basement membrane in the capsule. A significant decrease in the height of the epithelial layer of the proximal and distal convoluted tubules, expansion of the tubular lumen, and initial signs of fibrosis in the interstitial tissue were detected. A decrease in glomerular density was noted without changing the total number of nephrons. The results obtained prove that iron deficiency leads to the formation of a hypoxic-metabolic type of kidney damage, as well as the development of early tubulointerstitial remodeling.
References
Zhao S, Wang X, Zheng X, Liang X, Wang Z, Zhang J, et al. Iron deficiency exacerbates cisplatin- or rhabdomyolysis-induced acute kidney injury. Free Radic Biol Med. 2021;173:81-96. doi:10.1016/j.freeradbiomed.2021.07.025
Ikeda Y, Horinouchi Y, Hamano H, Hirayama T, Kishi S, Izawa-Ishizawa Y, et al. Dietary iron restriction alleviates renal tubulointerstitial injury induced by protein overload in mice. Sci Rep. 2017;7:10621. doi:10.1038/s41598-017-11089-0
Garrido P, Ribeiro S, Fernandes J, Vala H, Bronze-da-Rocha E, Rocha-Pereira P, et al. Iron-Hepcidin Dysmetabolism, Anemia and Renal Hypoxia, Inflammation and Fibrosis in the Remnant Kidney Rat Model. PLoS One. 2015;10(4):e0124048. doi:10.1371/journal.pone.0124048
Hanudel MR, Eisenga MF, Rappaport M, Chua K, Qiao B, Jung G, et al. Effects of dietary iron intake and chronic kidney disease on fibroblast growth factor 23 metabolism. Am J Physiol Renal Physiol. 2016;311:F1369-F1377. doi:10.1152/ajprenal.00281.2016
Veuthey T, Hoffmann D, Wessling-Resnick M. Impaired renal function and development in Belgrade rats. Am J Physiol Renal Physiol. 2014;306:F333-F343. doi:10.1152/ajprenal.00285.2013
Sun MY, Ollerenshaw SL, Wedgwood S, et al. Dietary-induced gestational iron deficiency and nephrogenesis. Reprod Fertil Dev. 2017;29(5):855-866. doi:10.1071/RD15358
Woodman AG, Mah RL, Kinney S, et al. Perinatal iron deficiency alters renal retinoic acid signaling and nephrogenesis. J Nutr Biochem. 2023;112:109227. doi:10.1016/j.jnutbio.2022.109227
Wlazlo E, Mehrad B, Morel L, Scindia Y. Iron Metabolism as a Driver of Renal Pathology. Front Med. 2021;8:643686. doi:10.3389/fmed.2021.643686
