SIGNIFICANCE OF EXPRESSION OF PD-L1 AND P53 PROTEINS IN HUMAN PAPILLOMAVIRUS-ASSOCIATED OROPHARYNGEAL SQUAMOUS CELL CARCINOMA
Keywords:
Oropharyngeal squamous cell carcinoma, human papillomavirus, immunohistochemistry, molecular marker, protein expression, p16INK4a, PD-L1, p53.Abstract
High-risk human papillomavirus (HPV), causes oropharyngeal squamous cell carcinoma (OSCC), where it is detected in approximately 70% of tumors arising from the lymphoid tissues of the tonsils or the base of the tongue.
The study included 76 patients with T1-4N0-3M0 (AJCC, 7th edition) OSCC, who received treatment at the Republican Specialized Scientific and Practical Medical Center of Oncology and Radiology and its Tashkent city branch in the period 2015-2020. An IHC evaluation of the expression of the three molecular markers p16INK4a, PD-L1, and p53 was performed in formalin-fixed and paraffin-embedded samples from 30 selected patients with OSCC. Overexpression of p16INK4a was accompanied by a positive PD-L1 reaction in 46% (6/13) and no positive expression of the p53 mutant type was observed. It is important to note that wild type p53 was identified as an exclusive type in only 1 case (3%) in combination with overexpression of p16INK4a. Establishing the characteristic features of the molecular profile of OSCC can help to better understand its biological characteristics of the tumor, moreover, it plays an important role in the prevention and effective treatment of diseases.
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